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Membership type: full

Mariela Bollati Bollati-Fogolín

Country of origin: Uruguay Currently in: Uruguay, Montevideo General field of specialization: Biological Systems and Organisms
Academic Background


2002 Doctorate Biological Systems and Organisms
Research and Profession

Current Research Activities

Biological Systems and Organisms

CELL CULTURE TECHNOLOGY: During the last years, our group has generated a variety of reporter cell lines with broader applications (type I IFN, redox biosensors, NF- kB, among others). These stable cell lines are being widely used to search and characterize substances that interfere with the type I IFN signaling pathways (Burgi et al, 2012 and Burgi et al, 2016), for the improvement of metabolism / productivity of cells with biotechnological interest (redox biosensors in collaboration with Dr Comini from the Redox Biology of Trypanosomes Lab, IP Montevideo and we have a manuscript in preparation), or for in vitro models of inflammation (NF- kB, Tiscornia et al, 2012; Mastropietro et al, 2015). ENVIRONMENTAL TOXICOLOGY: A wide variety of anthropogenic substances in the environment, known as endocrine disruptors (EDs), are able to alter the homeostasis of the endocrine system of organisms. In the field of endocrine disruption, the working hypothesis is that the increment of certain reproductive disorders are caused, at least in part, by an increased exposure to substances classified as EDs that are present in the environment. In this context, we focus on the design and development of in vitro and in vivo models for toxicological studies of EDs. For the in vitro approach, we are on the process to obtain a dual reporter cell line, to assess in a single assay the estrogenic or androgenic activity of a putative ED. For the in vivo studies, we proposed and validated the Oct4-GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, as a mammalian model to study the effects of EDs on the development of male germ cells. We introduced the transgenic Oct4/GFP mouse together with flow cytometry as a suitable tool to evaluate changes in male germ cells development and to identify early life exposures to EDCs (Porro et al, 2015 and one manuscript is in preparation). To accomplish the Project we had the financial support from ANII (2013-2015), one post-doctoral fellow ANII (R. Pagotto). In the frame of this project we are actively collaborating with Dr. Rodríguez (ISAL, CONICET-UNL, Santa Fe, Argentina).

Research Keywords: 
cell culture technology
flow cytometry
Environmental Toxicology

Publications resulting from Research: 

Last five years:

1- L. Malacrida; S Astrada, Arturo Briva, M Bollati-Fogolín, E Gratton, L Bagatolli. Spectral Phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar bodies-like structures. Biochim Biophys Acta. 2016; 1858(11):2625-2635.

2- Rolny IS, Tiscornia I, Racedo SM, Pérez PF, Bollati-Fogolín M. Lactobacillus delbrueckii subsp lactis CIDCA 133 modulates response of human epithelial and dendritic cells infected with Bacillus cereus. Benef Microbes. 2016, 26:1-12.

3- Bürgi M, Zapol’skii VA, Hinkelmann B, Köster M, Kaufmann DE, Sasse F, Hauser H, Etcheverrigaray M, Kratje R, Bollati-Fogolín M, Oggero M. Screening and characterization of molecules that modulate the biological activity of IFNs-I. J Biotechnol. 2016; 233:6-16.

4- García EP, Tiscornia I, Libisch G, Trajtenberg F, Bollati-Fogolín M, Rodríguez E, Noya V, Chiale C, Brossard N, Robello C, Santiñaque F, Folle G, Osinaga E, Freire T. MUC5B silencing reduces chemo-resistance of MCF-7 breast tumor cells and impairs maturation of dendritic cells. Int J Oncol. 2016 Mar 10. doi: 10.3892/ijo.2016.3434

5- Ubillos L, Freire T, Berriel E, Chiribao ML, Chiale C, Festari MF, Medeiros A, Mazal D, Rondán M, Bollati-Fogolín M, Rabinovich GA, Robello C, Osinaga E. Trypanosoma cruzi extracts elicits protective immune response against chemically induced colon and mammary cancers. Int J Cancer. 2016 Apr 1;138(7):1719-31.

6- Leon IE, Cadavid Vargas JF, Tiscornia I, Porro V, Castelli S, Desideri A, Bollati-Fogolín M, Etcheverry SB. Oxidovanadium(IV) Complexes with chrysin and silibinin. Anticancer activity and mechanisms of action in a human colon adenocarcinoma model. J Biol Inorg Chem. 2015 Oct;20(7):1175-91. doi: 10.1007/s00775-015-1298-7.

7- Núñez de Villavicencio-Díaz T, Ramos Gómez Y, Oliva Argüelles B, Fernández Masso JR, Rodríguez-Ulloa A, Cruz García Y, Guirola-Cruz O, Perez-Riverol Y, Javier González L, Tiscornia I, Victoria S, Bollati-Fogolín M, Besada Pérez V, Guerra Vallespi M. Data for comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells. Data Brief. 2015 Jul 8;4:468-73. doi: 10.1016/j.dib.2015.06.024.

8- Negrotto S, Mena HA, Ure AE, Jaquenod De Giusti C, Bollati-Fogolín M, Vermeulen EM, Schattner M, Gómez RM. Human Plasmacytoid Dendritic Cells Elicited Different Responses after Infection with Pathogenic and Nonpathogenic Junin Virus Strains. J Virol. 2015 Jul 15;89(14):7409-13. doi: 10.1128/JVI.01014-15.

9- Porro V, Pagotto R, Harreguy MB, Ramírez S, Crispo M, Santamaría C, Luque EH, Rodríguez HA, Bollati-Fogolín M. Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry. J Steroid Biochem Mol Biol. 2015 Jul 4. pii: S0960-0760(15)30006-6. doi: 10.1016/j.jsbmb.

10- Salzman V, Porro V, Bollati-Fogolín M, Aguilar PS. Quantitation of yeast cell-cell fusion using multicolor flow cytometry. Cytometry A. 2015 May 28. doi: 10.1002/cyto.a.22701.

11- Núñez de Villavicencio-Díaz T, Ramos Gómez Y, Oliva Argüelles B, Fernández Masso JR, Rodríguez-Ulloa A, Cruz García Y, Guirola-Cruz O, Perez-Riverol Y, Javier González L, Tiscornia I, Victoria S, Bollati-Fogolín M, Besada Pérez V, Guerra Vallespi M. Comparative proteomics analysis of the antitumor effect of CIGB-552 peptide in HT-29 colon adenocarcinoma cells. J Proteomics. 2015 May 24;126:163-171. doi: 10.1016/j.jprot.2015.05.024.

12- Mastropietro G, Tiscornia I, Perelmuter K, Astrada S, Bollati-Fogolín M. HT-29 and Caco-2 reporter cell lines for functional studies of nuclear factor kappa B activation. Mediators Inflamm. 2015;2015:860534. doi: 10.1155/2015/860534.

13- León IE, Porro V, Astrada S, Egusquiza MG, Cabello CI, Bollati-Fogolin M, Etcheverry SB. Polyoxometalates as antitumor agents: Bioactivity of a new polyoxometalate with copper on a human osteosarcoma model. Chem Biol Interact. 2014 Oct 22;222C:87-96. doi: 10.1016/j.cbi.2014.10.012.

14- Vallespí MG, Pimentel G, Cabrales-Rico A, Garza J, Oliva B, Mendoza O, Gomez Y, Basaco T, Sánchez I, Calderón C, Rodriguez JC, Markelova MR, Fichtner I, Astrada S, Bollati-Fogolín M, Garay HE, Reyes O. Antitumor efficacy, pharmacokinetic and biodistribution studies of the anticancer peptide CIGB-552 in mouse models. J Pept Sci. 2014 Nov;20(11):850-9. doi: 10.1002/psc.2676.

15- Beedessee G, Ramanjooloo A, Tiscornia I, Cresteil T, Raghothama S, Arya D, Rao S, Gowd KH, Bollati-Fogolin M, Marie DE. Evaluation of hexane and ethyl acetate extracts of the sponge Jaspis diastra collected from Mauritius Waters on HeLa cells. J Pharm Pharmacol. 2014 Sep;66(9):1317-27. doi: 10.1111/jphp.12256. Epub 2014 Apr 24.

16- Fernández-Calero T, Astrada S, Alberti A, Horjales S, Arnal JF, Rovira C, Bollati-Fogolín M, Flouriot G, Marin M. The transcriptional activities and cellular localization of the human estrogen receptor alpha are affected by the synonymous Ala87 mutation. J Steroid Biochem Mol Biol. 2014 Sep;143:99-104. doi: 10.1016/j.jsbmb.2014.02.016. Epub 2014 Mar 4.

17- Domínguez MF, Koziol U, Porro V, Costábile A, Estrade S, Tort J, Bollati-Fogolin M, Castillo E. A new approach for the characterization of proliferative cells in cestodes. Exp Parasitol. 2014 Mar;138:25-9. doi: 10.1016/j.exppara.2014.01.005.

18- Leon IE, Porro V, Di Virgilio AL, Naso LG, Williams PAM, Bollati-Fogolín M and Etcheverry SB. Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells. J Biol Inorg Chem, 2013, DOI 10.1007/s00775-013-1061-x.

19- Mazal D, Lo-Man R, Bay S, Pritsch O, Dériaud E, Ganneau C, Medeiros A, Ubillos L, Obal G, Berois N, Bollati-Fogolin M, Leclerc C, Osinaga E. Monoclonal antibodies toward different Tn-amino acid backbones display distinct recognition patterns on human cancer cells. Implications for effective immuno-targeting of cancer. Cancer Immunol Immunother. 2013; 62(6):1107-22. doi: 10.1007/s00262-013-1425-7.

20- Noya V, Bay S, Festari MF, García E, Rodriguez E, Chiale C, Ganneau C, Baleux F, Astrada S, Bollati–Fogolín M, Osinaga E and Freire T. Mucin-like peptides from Echinococcus granulosus induce antitumor activity. Int J Oncol. 2013; 43(3):775-84. doi: 10.3892/ijo.2013.2000.

21- Chenoll E, Codoñer FM, Silva A, Ibáñez A, Martinez-Blanch JF, Bollati-Fogolín M, Crispo M, Ramírez S, Sanz Y, Ramón D and Genovés S. Genomic Sequence and Pre-Clinical Safety Assessment of Bifidobacterium longum CECT 7347, a Probiotic able to Reduce the Toxicity and Inflammatory Potential of Gliadin-Derived Peptides. J Prob Health 2013, 1: 106. doi: 10.4172/ jph.1000106

22- Leon IE, Di Virgilio AL, Porro V, Muglia CI, Naso LG, Williams PAM, Bollati-Fogolín M and Etcheverry SB. Antitumor properties of a vanadyl(IV) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: role of the oxidative stress and apoptosis. Dalton Trans. 2013; 42(33):11868-80. doi: 10.1039/c3dt50524c.

23- Fernández Massó JR, Oliva Argüelles B, Tejeda Y, Astrada S, Garay H, Reyes O, Delgado-Roche L, Bollati-Fogolín M, Vallespí MG. The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells. J Amino Acids. 2013;2013:251398.

24- Grompone G, Martorell P, Llopis S, González N, Genovés S, Mulet AP, Fernández-Calero T, Tiscornia I, Bollati-Fogolín M, Chambaud I, Foligné B, Montserrat A, Ramón D. Anti-Inflammatory Lactobacillus rhamnosus CNCM I-3690 Strain Protects against Oxidative Stress and Increases Lifespan in Caenorhabditis elegans. PLoS One. 2012;7(12):e52493.

25- Tiscornia I., Sánchez-Martins V., Hernández A., Bollati-Fogolín M. Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells. J Immunol Methods. 2012, 384(1-2):164-70.

26- Bürgi M., Prieto C., Etcheverrigaray M., Kratje R., Oggero M., Bollati-Fogolín M. WISH cell line: From the antiviral system to a novel reporter gene assay to test the potency of human IFN-α and IFN-β. J Immunol Methods. 2012, 381(1-2):70-4.

27- Hernández P., Cabrera M., Lavaggi M.L., Celano L., Tiscornia I., Rodrigues da Costa T., Thomson L., Bollati-Fogolín M., Miranda A.L., Moreira-Lima L., Barreiro E., González M. and Cerecetto H. Discovery of new orally effective analgesic and anti-inflammatory hybrid furoxanyl n-acylhydrazone derivatives. Bioorg Med Chem. 2012; 20(6): 2158-71.

28- Bürgi M., Prieto C., Oggero M., Bollati-Fogolín M., Etcheverrigaray M., Kratje R. New reporter cell clones to determine the biological activity of human type I interferons. BMC Proceedings 2011, 5 (Suppl8): P4.

29- Pils, M; Bleich, A; Fasnacht, N; Bollati-Fogolin, M; Schippers, A; Rozell, B; Müller, W. Commensal gut flora reduces susceptibility to experimentally induced colitis via T-cell derived Interleukin-10. Inflamm Bowel Dis. 2011; 17(10): 2038-46.

1- Bollati-Fogolín, M. and Comini, M. (2008) Clonagem e expressäo de proteinas heterologas em células animais. En: Tecnologia do Cultivo de Céluas Animais: de Biofármacos a Terapia Genica. Castilho, Moraes, Augusto (Eds), Published by Editora Roca Ltda, São Paulo, Brasil, 3: 40-76.
2- Bollati-Fogolín, M. and Comini, M (2007), Cloning and expression of heterologous proteins in animal cells. En: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Castilho, Moraes, Augusto, Butler (Eds), Published by Taylor & Francis Group. 3:39-73.
3- Ceaglio N., Bollati-Fogolín M., Oggero M., Etcheverrigaray M., Kratje R (2014). Chapter 13: High cell density cultivation processes. In Animal Cell Biotechnology in Biologics Production, Eds Wagner R and Hauser H. De Gruyter. Berlín, Alemania (ISBN 3110278863, 9783110278866), 2014, pp. 427 – 454.

Current profession

Current professional activities type: 
Head of Cell Biology Unit at Institut Pasteur de Montevideo, Uruguay. Profesor of Cell Biology, School of Biotechnology, Ort University, Montevideo, Uruguay.

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